abstract：:The terminal branches of motoneurons can be excited by electrical current pulses delivered via needles inserted intramuscularly. Observations are reported which indicate that tungsten semi-microelectrodes suitably positioned in a muscle belly can excite antidromically a large proportion of the alpha-motoneurons of tha...

journal_title：Journal of neuroscience methods

authors： Muir RB,Lemon RN

更新日期：1983-05-01 00:00:00

abstract：:The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...

journal_title：Journal of neuroscience methods

authors： Glaser EM,Tagamets M,McMullen NT,Van der Loos H

更新日期：1983-05-01 00:00:00

abstract：:A procedure is described by which the solid silver chromate precipitate present in Golgi-impregnated neurons is reduced by a photographic developer into metallic silver grains or particles. The remaining silver chromate is subsequently removed, and the fine structure and synaptic relationships of the impregnated neuro...

journal_title：Journal of neuroscience methods

authors： Wouterlood FG,Nederlof J,Paniry S

更新日期：1983-04-01 00:00:00

abstract：:A technique for the preparation and recording of mammalian supraoptic nucleus neurosecretory neurons maintained in vitro through perfusion of the anterior cerebral artery is described. A slice of basal diencephalon measuring approximately 8 X 8 X 2 mm that includes the pituitary stalk and medial preoptic-anterior peri...

journal_title：Journal of neuroscience methods

authors： Bourque CW,Renaud LP

更新日期：1983-03-01 00:00:00

abstract：:A protocol has been established for the staining of myelin in frozen sections. While the new method is relatively fast and simple, it eliminates the problems routinely encountered with myelin stains such as blotchiness and uneven staining. Modifications were introduced into the procedures in order to obtain excellent ...

journal_title：Journal of neuroscience methods

authors： Hutchins B,Weber JT

更新日期：1983-03-01 00:00:00

abstract：:The cytochemical localization of acetylcholinesterase activity at motor endplates is commonly employed prior to electron microscopic examination. Methods which involve the use of thiocholine esters often lead to a loss of resolution due to the spread to surrounding tissues of an extremely dense reaction product. A met...

journal_title：Journal of neuroscience methods

authors： Strum JM,Hall-Craggs EC

更新日期：1982-11-01 00:00:00

abstract：:The calculation of volume fractions in nervous tissue is a method often used in neuroanatomy. In ontogenetic studies and problems concerning aging, as well as after experimental procedures, volume fractions are calculated in order to quantify the results. Grey level index (GLI) and grey cell coefficient (GCC) are diff...

journal_title：Journal of neuroscience methods

authors： Wree A,Schleicher A,Zilles K

更新日期：1982-07-01 00:00:00

abstract：:A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibitio...

journal_title：Journal of neuroscience methods

authors： Long JB,Youngblood WW,Kizer JS

更新日期：1982-07-01 00:00:00

abstract：:A technique is described for dark-field and light--dark-field illumination of autoradiographic preparations for photomacrogra. A right-angle prism is employed as a substrate through which illumination is introduced to the specimen. The technique permits uniform illumination of large fields of view, and also minimizes ...

journal_title：Journal of neuroscience methods

authors： Updyke BV

更新日期：1982-05-01 00:00:00

abstract：:Adult rat spinal cords were perfused intracardially with aldehyde fixatives containing either sodium cacodylate or sodium phosphate buffer for EM studies. The perfusion flow rate of 24 cc/min for the first 3 min and 33 cc/min for completion of prefixation was found to be optimal. Ultrastructural examination of the L-1...

journal_title：Journal of neuroscience methods

authors： Hill PK,de la Torre JC,Thompson SM,Bullock DF,Rosenfield-Wessells S

更新日期：1982-01-01 00:00:00

abstract：:In the last few years quite powerful complete computer systems have become available that fill many laboratory needs at low cost (US $3000-10,000). This development is based on the use of microcomputers, which now have capabilities that were previously associated with minicomputers. Due to the rapidly expanding use of...

journal_title：Journal of neuroscience methods

authors： Flaming DG

更新日期：1982-01-01 00:00:00

abstract：:A reliable technique for purification of crude tissue extracts and analysis for TRH by HPLC, TLC, radioimmunoassay and bioassay is presented. We conclude that authentic TRH is present throughout the mammalian brain and accounts for much of the TRH-like immunoreactivity in several peripheral organs, but that it is not ...

journal_title：Journal of neuroscience methods

authors： Busby WH Jr,Youngblood WW,Humm J,Kizer JS

更新日期：1981-12-01 00:00:00

abstract：:Exceptionally clear Golgi-Nissl sections of 300 micron thickness have been morphometrically studied by light microscopy using oil immersion objectives. The clarity results from a new variation of a staining procedure that combines Golgi and Nissl images in one section. A viewing technique has been developed that permi...

journal_title：Journal of neuroscience methods

authors： Glaser EM,Van der Loos H

更新日期：1981-08-01 00:00:00

abstract：:A Lucite plate reconstruction method is described which, when combined with HRP histochemistry, provides an excellent means of visualizing, in 3-dimensional fashion, the functional organization of neuronal populations. Color photomicrographs of representative serial sections were made through the baboon oculomotor nuc...

journal_title：Journal of neuroscience methods

authors： Augustine JR

更新日期：1981-06-01 00:00:00

abstract：:This paper presents an atlas of the rat brain based on horizontal sections stained for acetylcholinesterase (AChE). Fourteen labeled photographs from sections at 0.5 mm intervals are presented. The atlas is intended for use with male rats 250-300 g in weight. It was constructed using the flat-skull position (lambda an...

journal_title：Journal of neuroscience methods

authors： Paxinos G,Watson CR,Emson PC

更新日期：1980-12-01 00:00:00

abstract：:An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a spec...

journal_title：Journal of neuroscience methods

authors： Mattson DH,Roos RP,Arnason BG

更新日期：1980-10-01 00:00:00

abstract：:A method is described for achieving gaze control in cats, by rewarding them for pushing against a lever with their tongue, only when a light-emitting diode (LED) was flickering. Since the state of the LED was changed sequentially from OFF to ON to flickering and the duration of flickering was short, the cats were requ...

journal_title：Journal of neuroscience methods

authors： Hamada T

更新日期：1980-10-01 00:00:00

abstract：:The social interaction test in rats provides a method for detecting anxiolytic activity that does not use food or water deprivation, or electric shock, and therefore obviates difficulties of interpretation that might arise from drug-induced changes in motivation. Since social interaction is measured under more than on...

journal_title：Journal of neuroscience methods

authors： File SE

更新日期：1980-06-01 00:00:00

abstract：:Analysis of the antigenic components of synaptic membranes presents several problems caused by detergents required to solubilize the proteins. These problems involve detection of the reaction between antibodies and proteins in the detergent extract as well as characterization of the individual antigens after further p...

journal_title：Journal of neuroscience methods

authors： Mahadik SP,Korenovsky A,Huang YY,Graf L,Rapport MM

更新日期：1980-04-01 00:00:00

abstract：:A procedure is described for chronic single-unit recording from monkey auditory cortex. The cortex is approached in a vertical stereotaxic plane and subsequent histology is performed on tissue sections made in the same plane. The procedure maximizes the probability of locating and identifying auditory cortical areas d...

journal_title：Journal of neuroscience methods

authors： Pfingst BE,O'Connor TA

更新日期：1980-02-01 00:00:00

abstract：:With the system described specific features in a histological section such as neuronal degeneration, specific cell types, or the silver grains of autoradiography can be accurately localized. It is simple to operate, and allows for data storage and retrieval. The system consists of a Zeiss Universal microscope equipped...

journal_title：Journal of neuroscience methods

authors： Forbes DJ,Petry RW

更新日期：1979-03-01 00:00:00